Molecular Typing of Bois Noir Phytoplasma Strains in the Chianti Classico Area (Tuscany, Central Italy) and Their Association with Symptom Severity in Vitis vinifera 'Sangiovese'.
Identifieur interne : 000241 ( Main/Exploration ); précédent : 000240; suivant : 000242Molecular Typing of Bois Noir Phytoplasma Strains in the Chianti Classico Area (Tuscany, Central Italy) and Their Association with Symptom Severity in Vitis vinifera 'Sangiovese'.
Auteurs : R. Pierro [Italie] ; A. Passera [Italie] ; A. Panattoni [Italie] ; P. Casati [Italie] ; A. Luvisi [Italie] ; D. Rizzo [Italie] ; P A Bianco [Italie] ; F. Quaglino [Italie] ; A. Materazzi [Italie]Source :
- Phytopathology [ 0031-949X ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ADN bactérien, Phytoplasma.
- microbiologie : Maladies des plantes, Vitis.
- métabolisme : ADN bactérien.
- Italie, Phylogenèse, Typage moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Bacterial.
- chemical , metabolism : DNA, Bacterial.
- classification : Phytoplasma.
- genetics : Phytoplasma.
- microbiology : Plant Diseases, Vitis.
- Italy, Molecular Typing, Phylogeny.
Abstract
Bois noir (BN) is the most widespread disease of the grapevine yellows complex in the Euro-Mediterranean area. BN is caused by 'Candidatus Phytoplasma solani' (BNp), transmitted from herbaceous plants to grapevine by polyphagous insect vectors. In this study, genetic diversity among BNp strains and their prevalence and possible association with grapevine symptom severity were investigated in a Sangiovese clone organic vineyard in the Chianti Classico area (Tuscany). Field surveys over 2 years revealed a range of symptom severity on grapevine and an increase of BN incidence. A TaqMan allelic discrimination assay detected only tufB type b among BNp strains, suggesting the prevalence of the bindweed-related ecology. Nucleotide sequence analyses of vmp1 and stamp genes identified 12 vmp1 and 16 stamp sequence variants, showing an overall positive selection for such genes. The prevalent genotype was Vm43/St10, reported for the first time in this study and closely related to strains identified only in the French Eastern Pyrenees. BNp strains identified in the examined vineyard and mostly grouped in separate bindweed-related phylogenetic clusters showed statistically significant differences in their distribution in grapevines exhibiting distinct symptom severity. These results suggest the possible occurrence of a range of virulence within BNp strain populations in the Chianti Classico area.
DOI: 10.1094/PHYTO-06-17-0215-R
PubMed: 29027886
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Casati</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Luvisi, A" sort="Luvisi, A" uniqKey="Luvisi A" first="A" last="Luvisi">A. Luvisi</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Rizzo, D" sort="Rizzo, D" uniqKey="Rizzo D" first="D" last="Rizzo">D. Rizzo</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Bianco, P A" sort="Bianco, P A" uniqKey="Bianco P" first="P A" last="Bianco">P A Bianco</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Quaglino, F" sort="Quaglino, F" uniqKey="Quaglino F" first="F" last="Quaglino">F. Quaglino</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Materazzi, A" sort="Materazzi, A" uniqKey="Materazzi A" first="A" last="Materazzi">A. Materazzi</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29027886</idno><idno type="pmid">29027886</idno><idno type="doi">10.1094/PHYTO-06-17-0215-R</idno><idno type="wicri:Area/Main/Corpus">000286</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000286</idno><idno type="wicri:Area/Main/Curation">000286</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000286</idno><idno type="wicri:Area/Main/Exploration">000286</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular Typing of Bois Noir Phytoplasma Strains in the Chianti Classico Area (Tuscany, Central Italy) and Their Association with Symptom Severity in Vitis vinifera 'Sangiovese'.</title><author><name sortKey="Pierro, R" sort="Pierro, R" uniqKey="Pierro R" first="R" last="Pierro">R. Pierro</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Passera, A" sort="Passera, A" uniqKey="Passera A" first="A" last="Passera">A. Passera</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Panattoni, A" sort="Panattoni, A" uniqKey="Panattoni A" first="A" last="Panattoni">A. Panattoni</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Casati, P" sort="Casati, P" uniqKey="Casati P" first="P" last="Casati">P. Casati</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Luvisi, A" sort="Luvisi, A" uniqKey="Luvisi A" first="A" last="Luvisi">A. Luvisi</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Rizzo, D" sort="Rizzo, D" uniqKey="Rizzo D" first="D" last="Rizzo">D. Rizzo</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Bianco, P A" sort="Bianco, P A" uniqKey="Bianco P" first="P A" last="Bianco">P A Bianco</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Quaglino, F" sort="Quaglino, F" uniqKey="Quaglino F" first="F" last="Quaglino">F. Quaglino</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author><author><name sortKey="Materazzi, A" sort="Materazzi, A" uniqKey="Materazzi A" first="A" last="Materazzi">A. Materazzi</name><affiliation wicri:level="4"><nlm:affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia</wicri:regionArea><orgName type="university">Université de Pise</orgName><placeName><settlement type="city">Pise</settlement><region type="region" nuts="2">Toscane</region></placeName></affiliation></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Bacterial (genetics)</term><term>DNA, Bacterial (metabolism)</term><term>Italy (MeSH)</term><term>Molecular Typing (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytoplasma (classification)</term><term>Phytoplasma (genetics)</term><term>Plant Diseases (microbiology)</term><term>Vitis (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>ADN bactérien (métabolisme)</term><term>Italie (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Phylogenèse (MeSH)</term><term>Phytoplasma (classification)</term><term>Phytoplasma (génétique)</term><term>Typage moléculaire (MeSH)</term><term>Vitis (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Phytoplasma</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytoplasma</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Phytoplasma</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Italy</term><term>Molecular Typing</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Italie</term><term>Phylogenèse</term><term>Typage moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bois noir (BN) is the most widespread disease of the grapevine yellows complex in the Euro-Mediterranean area. BN is caused by 'Candidatus Phytoplasma solani' (BNp), transmitted from herbaceous plants to grapevine by polyphagous insect vectors. In this study, genetic diversity among BNp strains and their prevalence and possible association with grapevine symptom severity were investigated in a Sangiovese clone organic vineyard in the Chianti Classico area (Tuscany). Field surveys over 2 years revealed a range of symptom severity on grapevine and an increase of BN incidence. A TaqMan allelic discrimination assay detected only tufB type b among BNp strains, suggesting the prevalence of the bindweed-related ecology. Nucleotide sequence analyses of vmp1 and stamp genes identified 12 vmp1 and 16 stamp sequence variants, showing an overall positive selection for such genes. The prevalent genotype was Vm43/St10, reported for the first time in this study and closely related to strains identified only in the French Eastern Pyrenees. BNp strains identified in the examined vineyard and mostly grouped in separate bindweed-related phylogenetic clusters showed statistically significant differences in their distribution in grapevines exhibiting distinct symptom severity. These results suggest the possible occurrence of a range of virulence within BNp strain populations in the Chianti Classico area.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29027886</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>03</Day></DateCompleted><DateRevised><Year>2018</Year><Month>05</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>108</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Molecular Typing of Bois Noir Phytoplasma Strains in the Chianti Classico Area (Tuscany, Central Italy) and Their Association with Symptom Severity in Vitis vinifera 'Sangiovese'.</ArticleTitle><Pagination><MedlinePgn>362-373</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-06-17-0215-R</ELocationID><Abstract><AbstractText>Bois noir (BN) is the most widespread disease of the grapevine yellows complex in the Euro-Mediterranean area. BN is caused by 'Candidatus Phytoplasma solani' (BNp), transmitted from herbaceous plants to grapevine by polyphagous insect vectors. In this study, genetic diversity among BNp strains and their prevalence and possible association with grapevine symptom severity were investigated in a Sangiovese clone organic vineyard in the Chianti Classico area (Tuscany). Field surveys over 2 years revealed a range of symptom severity on grapevine and an increase of BN incidence. A TaqMan allelic discrimination assay detected only tufB type b among BNp strains, suggesting the prevalence of the bindweed-related ecology. Nucleotide sequence analyses of vmp1 and stamp genes identified 12 vmp1 and 16 stamp sequence variants, showing an overall positive selection for such genes. The prevalent genotype was Vm43/St10, reported for the first time in this study and closely related to strains identified only in the French Eastern Pyrenees. BNp strains identified in the examined vineyard and mostly grouped in separate bindweed-related phylogenetic clusters showed statistically significant differences in their distribution in grapevines exhibiting distinct symptom severity. These results suggest the possible occurrence of a range of virulence within BNp strain populations in the Chianti Classico area.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pierro</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Passera</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Panattoni</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Casati</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luvisi</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rizzo</LastName><ForeName>D</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bianco</LastName><ForeName>P A</ForeName><Initials>PA</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Quaglino</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Materazzi</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>First, third, fifth, and ninth authors: Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; second, fourth, seventh, and eighth authors: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, University of Milan, via Celoria 2, 20133 Milano, Italy; fifth author: Department of Biological and Environmental Sciences and Technologies-University of Salento, via Provinciale Monteroni, 73100 Lecce, Italy; and sixth author: Regional Phytosanitary Service, Laboratory of Phytopathological Diagnostics and Molecular Biology, via Ciliegiole 99, 51100 Pistoia, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>01</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007558" MajorTopicYN="N">Italy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058889" MajorTopicYN="N">Molecular Typing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045582" MajorTopicYN="N">Phytoplasma</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">membrane protein</Keyword><Keyword MajorTopicYN="N">multiple gene typing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>10</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>10</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29027886</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-06-17-0215-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country><region><li>Toscane</li></region><settlement><li>Pise</li></settlement><orgName><li>Université de Pise</li></orgName></list><tree><country name="Italie"><region name="Toscane"><name sortKey="Pierro, R" sort="Pierro, R" uniqKey="Pierro R" first="R" last="Pierro">R. Pierro</name></region><name sortKey="Bianco, P A" sort="Bianco, P A" uniqKey="Bianco P" first="P A" last="Bianco">P A Bianco</name><name sortKey="Casati, P" sort="Casati, P" uniqKey="Casati P" first="P" last="Casati">P. Casati</name><name sortKey="Luvisi, A" sort="Luvisi, A" uniqKey="Luvisi A" first="A" last="Luvisi">A. Luvisi</name><name sortKey="Materazzi, A" sort="Materazzi, A" uniqKey="Materazzi A" first="A" last="Materazzi">A. Materazzi</name><name sortKey="Panattoni, A" sort="Panattoni, A" uniqKey="Panattoni A" first="A" last="Panattoni">A. Panattoni</name><name sortKey="Passera, A" sort="Passera, A" uniqKey="Passera A" first="A" last="Passera">A. Passera</name><name sortKey="Quaglino, F" sort="Quaglino, F" uniqKey="Quaglino F" first="F" last="Quaglino">F. Quaglino</name><name sortKey="Rizzo, D" sort="Rizzo, D" uniqKey="Rizzo D" first="D" last="Rizzo">D. Rizzo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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